Red signal from loading control ab8226 observed at 42 kda lanes 5 and 6.
Cdk2 antibody cell signaling.
Inhibitory phosphorylation occurs on thr14 and tyr15 1.
Recommended fluorochrome conjugated anti rabbit secondary antibodies.
Cyclin dependent kinase 2 p33cdk2 is an important component of the cell cycle machinery.
Antibody dilution buffer 1x pbs 1 bsa 0 3 triton x 100.
Wild type hap1 cell lysate 20 µg lanes 2 4 and 6.
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding thr160 of human cdk2.
Cdk2 knockout hap1 cell lysate 20 µg lanes 1 and 2.
Mix well then add 0 1 g bsa mix.
Immunoprecipitation of cdk12 from pc 3 cell extracts using normal rabbit igg 2729 lane 2 or cdk12 antibody lane 3.
Inhibitory phosphorylation occurs on thr14 and tyr15 1.
Like p34cdc2 kinase activity is regulated by phosphorylation state as well as association with a cyclin subunit and a cdk inhibitor.
Lanes 1 3 and 5.
An antibody shouldn t be one of the variables in your experiment.
Green signal from target ab32147 observed at 34 kda lanes 3 and 4.
Cdk2 cell division protein kinase 2 cyclin dependent kinase 2 cdc2 related protein kinase cell devision kinase 2 p33 protein kinase p33 cdk2 immunogen.
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Inhibitory phosphorylation occurs on thr14 and tyr15 1.
Like p34cdc2 kinase activity is regulated by phosphorylation state as well as association with a cyclin subunit and a cdk inhibitor.
Cyclin dependent kinase 2 p33cdk2 is an important component of the cell cycle machinery.
Lane 1 is 10 input.
It does not cross react with other phosphorylated cyclin dependent kinases.
Kinase activity associated with cyclin a cdc2 is found only in g2 phase.
Ab32147 was shown to specifically react with cdk2 when cdk2 knockout samples were used.
Anti rabbit igg h l f ab 2 fragment alexa fluor 488 conjugate 4412.
Antibodies are purified by protein a and peptide affinity chromatography.
Merged red and green signal.
The antibody weakly cross reacts with cdc2 phosphorylated at thr161.
Like p34cdc2 kinase activity is regulated by phosphorylation state as well as association with a cyclin subunit and a cdk inhibitor.
To prepare 10 ml add 30 µl triton x 100 to 10 ml 1x pbs.
Cdk2 antibodies cdk2 antibodies p33cdk2 associates with cyclin a in human cells.
